Western Blot Protocol

CONTENTS Introduction Lysate Preparation Sample Preparation SDS-PAGE Protein Transfer Immunoblotting Detection Buffers and Reagents INTRODUCTION Western blotting makes use of antibodies to determine particular person proteins inside a cell or tissue lysate. Antibodies bind to extremely particular sequences of amino acids, often known as epitopes. Because amino acid sequences fluctuate from protein to protein, western … Read more

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Genetic and phenotypic landscape of the mitochondrial genome in the Japanese population.

The genetic landscape of mitochondrial DNA (mtDNA) has been elusive. By analyzing mtDNA using the whole genome sequence (WGS) of Japanese individuals (n = 1928), we identified 2023 mtDNA variants and high-resolution haplogroups. Frequency spectra of the haplogroups were population-specific and were heterogeneous among geographic regions within Japan. Application of machine learning methods could finely classify the … Read more

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P53 research

What is the role of the p53 gene? It does this remains unclear, although the p53 gene is vital for tumor suppression. Upon genotoxic or oncogenic tests, increased p53 activity causes uncontrollable cell cycle arrest, senescence or apoptosis. The 3 cornerstones of those so-called triumvirate. Therefore, it’s long been believed that p53 inhibits tumorigenesis by … Read more

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