Cryopreservation is an environment friendly approach to retailer spermatozoa and is intently related to the high quality of sperm after the freeze-thaw course of. During freeze-thaw biking, excessive reactive oxygen species (ROS) are produced, and the results of ROS on boar sperm throughout cryopreservation haven’t been recognized. In this research, we evaluated the high quality of boar spermatozoa in several steps of cryopreservation (extension, cooling, and thawing for 30 min and 240 min) with or with out boar-sperm antioxidant (N-acetylcysteine (NAC)). The ROS ranges, sperm motility, plasma membrane integrity, mitochondrial exercise, sperm chromatin construction, ATP content material, and sperm apoptosis have been assayed.
After thawing, the ROS degree and sperm apoptosis have been considerably elevated, and the sperm motility, plasma membrane integrity, mitochondrial exercise, sperm chromatin construction, and ATP content material have been considerably impaired in contrast with these at the extension interval and cooling interval. Moreover, the addition of N-acetyl L-cysteine (NAC) reversed these adjustments. Coenzyme Q10 (CoQ10) has been reported to enhance bone density and the quantity of trabeculae in postmenopausal osteoporosis, however the mechanism stays to be elucidated. We aimed to analyze the results of CoQ10 on receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis and the underlying molecular mechanisms.
Tartrate-resistant acid phosphatase staining was carried out to detect osteoclasts. Cell viability was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, cell apoptosis was examined by movement cytometry, and the results of CoQ10 on protein and messenger RNA expression of mitochondrial apoptosis-associated proteins and osteoclast marker proteins have been measured by quantitative reverse transcription polymerase chain response and western blot, respectively. Furthermore, enzyme-linked immunosorbent assay was performed to investigate the actions of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT). RANKL considerably induced osteoclastogenesis in RAW264.7 cells, with the biggest effectivity at 50 ng/ml. CoQ10 had no important results on cell viability however it considerably elevated the percentages of cell apoptosis.
The freeze-thawing of boar spermatozoa impaired their motility, plasma membrane, mitochondrial exercise, sperm chromatin construction and apoptosis by producing excessive ROS. Thus, the downregulation of ROS degree by antioxidants, particularly the NAC, is vital for manufacturing frozen pig sperm to extend reproductive cells and livestock propagation, in addition to to enhance the software of frozen semen in pigs worldwide.
Involvement of totally different hemoprotein thiol teams of Oncorhynchus mykiss in cadmium toxicity
Cadmium is taken into account the seventh most poisonous heavy metallic as per ATSDR rating however its mechanism of toxicity is debated. Recently, we evaluated the results of this metallic on the erythrocyte of teleost fish (Oncorhynchus mykiss) main us to hypothesize that the pro-oxidant exercise of cadmium just isn’t linked to mitochondria however extra more likely to haemoglobin. In this context, the primary goal of this work was to detect the capacity of Cd to induce structural perturbation in haemoproteins that current totally different constructions and thus totally different purposeful properties and to determine what websites of interplay are primarily concerned.
The impact of Cd on the structural destabilization of the totally different haemoproteins was adopted spectrophometrically through their precipitation. In addition, the websites of interplay between the totally different haemoproteins and bivalent cadmium ions have been recognized by MIB server adopted by molecular docking/molecular dynamics simulations each in the dimeric and tetrameric associations. Cadmium doesn’t affect the autoxidation price of Mb, HbA and trout HbI. However, the presence of this metallic accelerates the precipitation course of in trout HbIV in a dose-dependent method.
Moreover, the presence of 1-10-50-250-500-1000 μM GSH, a chelating agent, reduces the capacity of cadmium to speed up the denaturation course of though it’s not capable of fully stop it. In order to elucidate the experimental outcomes, a computational investigations was carried out to determine the cadmium cation affinity for the studied haemoglobins and myoglobin, each of their dimeric and tetrameric types. As a end result, the highest affinity cadmium binding websites for fish HbIV are positioned at the interface between tetramer-tetramer affiliation, indicating that the cation can help supramolecular protein aggregations and induce complicated precipitation. For mammalian Hb, Mb and fish HbI computational investigation didn’t detect any website the place Cd might to induce such aggregation, according to the experimental outcomes.
Progressive parkinsonism attributable to mitochondrial impairment: Lessons from the MitoPark mouse mannequin
The cardinal pathophysiological discovering of Parkinson’s illness (PD) is a persistent, progressive degeneration of dopamine (DA) neurons in the substantia nigra, which is answerable for the motor and some of the non-motor symptomatology. While the major causes of nigrostriatal degeneration are hotly debated, appreciable proof helps a central position for impaired mitochondrial function. Postmortem evaluation of PD sufferers reveals impaired respiratory chains and elevated mutations of mitochondrial DNA (mtDNA), along with elevated markers of oxidative stress indicative of mitochondrial impairment.
 Mitochondria/Cytosol Fractionation Kit |
|
K2103-25 |
ApexBio |
25 assays |
EUR 251 |
|
Mitochondria/Cytosol Fractionation Kit |
|
K256-25 |
Biovision |
|
EUR 240 |
 Nuclear/Cytosol Fractionation Kit |
|
K2111-100 |
ApexBio |
100 assays |
EUR 544 |
|
Nuclear/Cytosol Fractionation Kit |
|
K266-100 |
Biovision |
|
EUR 539 |
 Mitochondria Fractionation Kit |
|
55R-1306 |
Fitzgerald |
25 assays |
EUR 296 |
|
Description: Mitochondria Fractionation Kit for use in the research laboratory |
|
Nuclear/Cytosol Fractionation Kit |
|
K2111-25 |
ApexBio |
25 assays |
EUR 251 |
|
Nuclear/Cytosol Fractionation Kit |
|
K266-25 |
Biovision |
|
EUR 262 |
 Acetylcholinesterase Activity Assay Kit - 100 Assays |
|
AR4001-unit |
BosterBio |
unit |
EUR 317 |
) MCB Glutathione Detection Kit (100 assays) |
|
30019 |
Biotium |
1KIT |
EUR 409 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
 Extract-EZ L1, Cytosol and Mitochondria Protein Extraction Kit |
|
BSP051 |
Bio Basic |
50Preps |
EUR 100.46 |
- Product category: Molecular Biology Kits/Protein - Extraction/Animal
|
) pCT-GFP-BAX (pCMV, Cytosol to Mitochondria, BAX Tag) |
|
CYTO115-PA-1 |
SBI |
10 ug |
EUR 684 |
- Category: Bioluminescent Imaging
|
|
pCT-GFP-BAX (pCMV, Cytosol to Mitochondria, BAX Tag) |
|
CYTO115-VA-1 |
SBI |
>2x10^6 IFUs |
EUR 781 |
- Category: Bioluminescent Imaging
|
) AccuGreen High Sensitivity dsDNA Quantitation Kit (100 assays) |
|
31066-T |
Biotium |
100kit |
EUR 133 |
|
Description: Minimum order quantity: 1 unit of 100kit |
) AccuGreenâ„¢ Broad Range dsDNA Quantitation Kit (100 assays) |
|
31069-T |
Biotium |
1kit |
EUR 124 |
|
Description: Minimum order quantity: 1 unit of 1kit |
 Nuclear Fractionation Kit |
|
55R-1307 |
Fitzgerald |
25 assays |
EUR 328 |
|
Description: Nuclear Fractionation Kit for use in the research laboratory |
) Annexin V, R-phycoerythrin conjugate (100 assays) |
|
9-29045 |
Biotium |
|
|
|
Description: Minimum order quantity: 1 unit of 500uL |
) Annexin V, Allophycocyanin crosslinked conjugate (100 assays) |
|
9-29057 |
Biotium |
|
|
|
Description: Minimum order quantity: 1 unit of 500uL |
) JC-1 Mitochondrial Membrane Potential Detection Kit (100 assays) |
|
30001 |
Biotium |
1KIT |
EUR 301 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
) AccuGreen High Sensitivity dsDNA Quantitation Solution (100 assays) |
|
31068-T |
Biotium |
100kit |
EUR 116 |
|
Description: Minimum order quantity: 1 unit of 100kit |
) AccuGreenâ„¢ Broad Range dsDNA Quantitation Solution (100 assays) |
|
31070-T |
Biotium |
1kit |
EUR 109 |
|
Description: Minimum order quantity: 1 unit of 1kit |
 Nuclear/Cytosolic Fractionation Kit |
|
AKR-171 |
Cell Biolabs |
20 preps |
EUR 276 |
|
Description: Cell Biolabs? Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours. The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting. |
|
Nuclear/Cytosolic Fractionation Kit |
|
AKR-172 |
Cell Biolabs |
100 preps |
EUR 664 |
|
Description: Cell Biolabs? Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours. The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting. |
 FractionPREP? Cell Fractionation Kit |
|
K2115-50 |
ApexBio |
50 assays |
EUR 599 |
|
FractionPREP? Cell Fractionation Kit |
|
K270-50 |
Biovision |
|
EUR 550 |
) NucView 488 and MitoView 633 Apoptosis Assay Kit (100 assays) |
|
30062 |
Biotium |
1KIT |
EUR 429 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
) NucView 488 and RedDot 2 Apoptosis & Necrosis Kit (100 assays) |
|
30072 |
Biotium |
1KIT |
EUR 382 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
) Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (100 assays) |
|
30008-2 |
Biotium |
1KIT |
EUR 383 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
) Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (100 assays) |
|
30011-2 |
Biotium |
1KIT |
EUR 383 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
 Mitochondria Isolation Kit |
|
AR0156 |
BosterBio |
1 kit |
EUR 106 |
 Cytosol/Particulate Separation Kit |
|
55R-1356 |
Fitzgerald |
50 assays |
EUR 551 |
|
Description: Cytosol/Particulate Separation Kit for use in the research laboratory |
|
Cytosol/Particulate Separation Kit |
|
K2112-50 |
ApexBio |
50 assays |
EUR 418 |
|
Cytosol/Particulate Separation Kit |
|
K267-50 |
Biovision |
|
EUR 403 |
) ViaFluor 488 SE Cell Proliferation Kit, Trial Size (1 vial, 100 assays) |
|
30086-T |
Biotium |
1KIT |
EUR 104 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
 LB-100 |
|
B4846-100 |
ApexBio |
100 mg |
EUR 860 |
 AIM-100 |
|
A3148-100 |
ApexBio |
100 mg |
EUR 1859 |
|
Description: AIM-100 is a small inhibitor of Ack1 tyrosine kinase with IC50 value of 24 nM [1]. AIM-100 mimicked ATP and inhibited the activity of Ack1 significantly and specifically. |
 Alleleustrious pmTFP1-Mitochondria fusion vector Mitochondria |
|
ABP-FP-TMIT |
Allele Biotech |
5 ug |
Ask for price |
- Product line: Plasmids
- Brand: Organelle Markers
|
 Alleleustrious pWasabi-Mitochondria fusion vector Mitochondria |
|
ABP-FP-WMIT |
Allele Biotech |
5 ug |
Ask for price |
- Product line: Plasmids
- Brand: Organelle Markers
|
 FractionPREP Cell Fractionation system |
|
55R-1359 |
Fitzgerald |
50 assays |
EUR 758 |
|
Description: Cell Fractionation system for use in the research laboratory |
 ReadiPrep™ Nuclear/Cytoplasmic Fractionation Kit |
|
60000 |
AAT Bioquest |
50 Tests |
EUR 306 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
 ReadiPrep™ Mitochondrial/Cytoplasmic Fractionation Kit |
|
60005 |
AAT Bioquest |
50 Tests |
EUR 306 |
- R-phrase: R20, R21, R22
- H-Phrase: H303, H313, H333
- Symbol for dangerous compounds: Xn
- UNSPEC Code: 12352200
|
 Mitochondria antibody |
|
10R-8363 |
Fitzgerald |
100 ul |
EUR 349 |
|
Description: Mouse monoclonal Mitochondria antibody |
 Cell Mitochondria Isolation Kit |
|
abx090630-50100tests |
Abbexa |
50-100 tests |
EUR 258 |
- Shipped within 5-10 working days.
|
 Tissue Mitochondria Isolation Kit |
|
abx090631-50100assays |
Abbexa |
50-100 assays |
EUR 258 |
- Shipped within 5-10 working days.
|
 Yeast Mitochondria Isolation Kit |
|
K259-50 |
Biovision |
|
EUR 419 |
 Mitochondria Activity Assay Kit |
|
KC310100 |
Biochain |
1 kit |
EUR 398 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 MTBK_20640-100 |
|
AR02-P0011-100 |
Abfrontier |
100ug |
EUR 1133 |
 hAlbumin FRET 100 |
|
AH1005-100 |
Mediomics |
1 Kit |
EUR 212 |
 hAlbumin TRF 100 |
|
AHTR1005-100 |
Mediomics |
1 Kit |
EUR 239 |
 hIgG TRF 100 |
|
HITR1011-100 |
Mediomics |
1 Kit |
EUR 239 |
 hInsulin FRET 100 |
|
IH1010-100 |
Mediomics |
1 Kit |
EUR 212 |
 hInsulin TRF 100 |
|
IHTR1010-100 |
Mediomics |
1 Kit |
EUR 239 |
) Etoposide (100 mM) |
|
1043-100 |
Biovision |
|
EUR 153 |
 hCRP FRET 100 |
|
CH1008-100 |
Mediomics |
1 Kit |
EUR 212 |
 hCRP TRF 100 |
|
CHTR1008-100 |
Mediomics |
1 Kit |
EUR 239 |
 rIgG FRET 100 |
|
RI1015-100 |
Mediomics |
1 Kit |
EUR 212 |
 hydroxyproline assay kit 96-assays |
|
QZBhypro1 |
Quickzyme |
1 plate 96 assays |
EUR 323 |
) Adipose Protein Fractionation Kit (water-soluble/insoluble) |
|
P523-2 |
101Bio |
NULL |
EUR 0 |
|
Adipose Protein Fractionation Kit (water-soluble/insoluble) |
|
P523-20 |
101Bio |
NULL |
EUR 0 |
|
Adipose Protein Fractionation Kit (water-soluble/insoluble) |
|
P523-80 |
101Bio |
NULL |
EUR 0 |
 Mitochondria Storage Buffer |
|
M1104-25 |
Biovision |
|
EUR 240 |
 ELISA Kit) Cow Cytosol aminopeptidase (LAP3) ELISA Kit |
|
abx513223-96tests |
Abbexa |
96 tests |
EUR 911 |
- Shipped within 5-12 working days.
|
 ELISA Kit) Human Cytosol aminopeptidase (LAP3) ELISA Kit |
|
abx513224-96tests |
Abbexa |
96 tests |
EUR 668 |
- Shipped within 5-12 working days.
|
 ELISA Kit) Mouse Cytosol aminopeptidase (LAP3) ELISA Kit |
|
abx513225-96tests |
Abbexa |
96 tests |
EUR 668 |
- Shipped within 5-12 working days.
|
 ELISA Kit) Pig Cytosol aminopeptidase (LAP3) ELISA Kit |
|
abx513226-96tests |
Abbexa |
96 tests |
EUR 911 |
- Shipped within 5-12 working days.
|
 ELISA Kit) Rat Cytosol aminopeptidase (LAP3) ELISA Kit |
|
abx513227-96tests |
Abbexa |
96 tests |
EUR 668 |
- Shipped within 5-12 working days.
|
 Mouse Lap3/ Cytosol aminopeptidase ELISA Kit |
|
E0851Mo |
Sunlong |
1 Kit |
EUR 571 |
 ELISA Kit) Human LAP3(Cytosol aminopeptidase) ELISA Kit |
|
EH9724 |
FN Test |
96T |
EUR 567.6 |
- Detection range: 0.156-10 ng/ml
|
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
 Human LAP3/ Cytosol aminopeptidase ELISA Kit |
|
E1428Hu |
Sunlong |
1 Kit |
EUR 571 |
 Membrane and Cytosol Protein Extraction Kit |
|
abx090627-100tests |
Abbexa |
100 tests |
EUR 321 |
- Shipped within 5-10 working days.
|
) QuantiChrom? LDH Cytotoxicity Assay Kit (C2LD-100) |
|
C2LD-100 |
Cusabio |
100 |
EUR 127.43 |
- Sample volume: 50-100ul
- Detection wavelength: 450nm
- Assay performance time: 1 to 4 hours.
|
|
Description: Quantitative determination of cytotoxicity based on cellular lactate dehydrogenase release into cell culture medium by colorimetric (565nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 2 U/L. Shelf life: 6 months. Shipping: ambient tem |
 Antibody) Cytosol Aminopeptidase (LAP3) Antibody |
|
20-abx126958 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
|
Cytosol Aminopeptidase (LAP3) Antibody |
|
20-abx113463 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
|
Cytosol Aminopeptidase (LAP3) Antibody |
|
20-abx141958 |
Abbexa |
-
EUR 370.00
-
EUR 606.00
-
EUR 300.00
|
|
- Shipped within 5-10 working days.
|
|
Cytosol Aminopeptidase (LAP3) Antibody |
|
abx146217-100ug |
Abbexa |
100 ug |
EUR 391 |
- Shipped within 5-10 working days.
|
|
Cytosol Aminopeptidase (LAP3) Antibody |
|
abx033307-400ul |
Abbexa |
400 ul |
EUR 523 |
- Shipped within 5-10 working days.
|
|
Cytosol Aminopeptidase (LAP3) Antibody |
|
abx033307-80l |
Abbexa |
80 µl |
EUR 286 |
- Shipped within 5-10 working days.
|
|
Cytosol Aminopeptidase (LAP3) Antibody |
|
20-abx006895 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
-
100 ul
-
200 ul
-
20 ul
-
50 ul
|
- Shipped within 5-10 working days.
|
|
Cytosol Aminopeptidase (LAP3) Antibody |
|
abx234694-100ug |
Abbexa |
100 ug |
EUR 481 |
- Shipped within 5-12 working days.
|
 Antibody) CD1b(100-1A5) Antibody |
|
BNC611044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF660R conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC401044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF640R conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC431044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF543 conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC471044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF647 conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC551044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF555 conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC051044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF405M conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC041044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF405S conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNUB1044-100 |
Biotium |
100uL |
EUR 209 |
|
Description: Primary antibody against CD1b(100-1A5), Concentration: 0.2mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC681044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF568 conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC701044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF770 conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC941044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF594 conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNCH1044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC801044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF680 conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNCAP1044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNCB1044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), Biotin conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC881044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF488A conjugate, Concentration: 0.1mg/mL |
|
CD1b(100-1A5) Antibody |
|
BNC811044-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against CD1b(100-1A5), CF680R conjugate, Concentration: 0.1mg/mL |
 Mouse IgM FRET 100 |
|
IM1009-100 |
Mediomics |
1 Kit |
EUR 212 |
) PBS Tablets (100 ml) |
|
2865-100 |
Biovision |
|
EUR 164 |
) ATP Solution (100 mM) |
|
2121-100 |
Biovision |
|
EUR 126 |
 FUNNEL, 100 MM, PP |
|
6120P-100 |
CORNING |
12/pk |
EUR 47 |
|
Description: Reusable Plastics; Reusable Funnels |
 Mouse IgG FRET 100 |
|
MIG1012-100 |
Mediomics |
1 Kit |
EUR 212 |
 GFP Antibody, 100 uL |
|
P601-100 |
101Bio |
NULL |
EUR 0 |
 100 BlotBot Reagent Reservoirs |
|
RRVR-100 |
Next Advance |
100pack |
EUR 177 |
|
Description: BlotBot reagent reservoirs will allow dispensing of unique reagents during processing. Pack of 100. |
-100) Rv0351 (GrpE)-100 |
|
AR02-P0003-100 |
Abfrontier |
100ug |
EUR 266 |
-100) Rv1009 (RpfB)-100 |
|
AR02-P0006-100 |
Abfrontier |
100ug |
EUR 1133 |
-100) Rv2450c (RpfE)-100 |
|
AR02-P0007-100 |
Abfrontier |
100ug |
EUR 1133 |
-100) Rv3875 (esxA)-100 |
|
AR02-P0008-100 |
Abfrontier |
100ug |
EUR 1133 |
-100) Rv2031c(HspX)-100 |
|
AR02-P0009-100 |
Abfrontier |
100ug |
EUR 1133 |
-100) Rv3131 (NfnB)-100 |
|
AR02-P0010-100 |
Abfrontier |
100ug |
EUR 1133 |
 Biotin TRF 100 |
|
BTTR-1006-100 |
Mediomics |
1 Kit |
EUR 239 |
) CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent) |
|
CASCL9-100A-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
) 100 bp DNA Ladder (100 bp ~ 1,500 bp, with loading dye) |
|
W0636-100 |
101Bio |
NULL |
EUR 0 |
) Apoptosis & Necrosis Quantification Kit (50 assays) |
|
30017 |
Biotium |
1KIT |
EUR 248 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
 AccuOrangeâ„¢ Protein Quantitation Kit, 2000 assays |
|
30071 |
Biotium |
1KIT |
EUR 288 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
 AccuOrangeâ„¢ Protein Quantitation Kit, 200 assays |
|
30071-T |
Biotium |
1KIT |
EUR 101 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
 soluble collagen assay kit 96-assays |
|
QZBcol1-1 |
Quickzyme |
1 plate 96 assays |
EUR 334 |
 hydroxyproline assay kit 2x 96-assays |
|
QZBhypro2 |
Quickzyme |
2 plates 192 assays |
EUR 590 |
 sensitive tissue hydroxyproline kit 96-assays |
|
QZBtishyp1 |
Quickzyme |
1 plate 96 assays |
EUR 401 |
 total collagen assay kit 96-assays |
|
QZBtotcol1 |
Quickzyme |
1 plate 96 assays |
EUR 334 |
 total protein assay kit 96-assays |
|
QZBtotprot1 |
Quickzyme |
1 plate 96 assays |
EUR 288 |
) OxiSelect AOPP Assay Kit (200 assays) |
|
STA-318 |
Cell Biolabs |
200 assays |
EUR 635 |
|
Description: Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of plasma proteins with chlorinated oxidants such as chloramines or hypochlorous acid. AOPP levels are elevated in patients with renal complications, diabetes mellitus, and atherosclerosis, as well as HIV-positive patients. Our OxiSelect AOPP Assay Kit provides a convenient method to assess oxidative stress. The kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control. |
 Frit Kit |
|
FRIT-KIT |
Next Advance |
1each |
EUR 124 |
|
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
 DiagNano Gold Nanoparticle Passive Conjugation Kit, 100 nm |
|
GPK-100 |
Creative Diagnostics |
1 kit |
EUR 715 |
 Human Anti- mitochondria autoantibodies ELISA Kit |
|
ELA-E0354h |
Lifescience Market |
96 Tests |
EUR 824 |
 Mammalian Mitochondria Isolation Kit for Tissue |
|
abx098857-50rxns |
Abbexa |
50 rxns |
EUR 411 |
- Shipped within 5-10 working days.
|
) Mitochondria Isolation Kit (Mammalian cells / tissues) |
|
P507L |
101Bio |
NULL |
EUR 0 |
 Column Packing Kit |
|
PACK-KIT |
Next Advance |
1pack |
EUR 1035 |
|
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
 Triton® X-100 Detergent |
|
B2394-100 |
Biovision |
|
EUR 109 |
 Antibody) bcl-2(100/D5) Antibody |
|
BNC610045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF660R conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC680045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF568 conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC430045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF543 conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC550045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF555 conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC040045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF405S conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC400045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF640R conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC050045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF405M conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC470045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF647 conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC700045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF770 conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC940045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF594 conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNCH0045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC800045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF680 conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC810045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF680R conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNCAP0045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNCB0045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), Biotin conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNC880045-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5), CF488A conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5) Antibody |
|
BNUB0045-100 |
Biotium |
100uL |
EUR 209 |
|
Description: Primary antibody against bcl-2(100/D5), Concentration: 0.2mg/mL |
 ERLENMEYER FLASK, 100 ML, PP |
|
4985P-100 |
CORNING |
6/pk |
EUR 54 |
|
Description: Reusable Plastics; Reusable Erl Flasks |
 ERLENMEYER FLASK, 100 ML, PMP |
|
4990P-100 |
CORNING |
6/pk |
EUR 57 |
|
Description: Reusable Plastics; Reusable Erl Flasks |
 S-100?? Protein, Bovine Brain |
|
P1275-100 |
Biovision |
|
EUR 403 |
 Bridge-It cAMP-PDE 100 |
|
PD1007-100 |
Mediomics |
1 Kit |
EUR 239 |
 Reflex Clips, 7mm , 100/Pk |
|
LC1001-100 |
GenDepot |
100/pk |
EUR 147 |
 AXYPET STARTER KIT 2 AP-10, AP-100 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS |
|
AP-STR-KIT-2 |
CORNING |
1/pk |
EUR 367 |
|
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
 Shmt1 ELISA Kit| Mouse Serine hydroxymethyltransferase, cytosol |
|
EF016166 |
Lifescience Market |
96 Tests |
EUR 689 |
 Pck1 ELISA Kit| Mouse Phosphoenolpyruvate carboxykinase, cytosol |
|
EF013117 |
Lifescience Market |
96 Tests |
EUR 689 |
) Total RNA Plus Mini Kit (100 prep) |
|
FATRK-P-100 |
Favorgen |
100 preps |
EUR 242 |
 PCR Mycoplasma Detection Kit |
|
M034-Kit |
TOKU-E |
Kit |
EUR 266 |
-100) Rv2769c (PE27 protein)-100 |
|
AR02-P0001-100 |
Abfrontier |
100ug |
EUR 479 |
) 2.5x12 cellogel 250µ (100) |
|
EHCA1200-ST01-100 |
Consort |
ea |
EUR 87 |
) 2.5x17 cellogel 200µ (100) |
|
EHCA1200-ST12-100 |
Consort |
ea |
EUR 123 |
) 4x17 cellogel 250µ (100) |
|
EHCA1200-ST21-100 |
Consort |
ea |
EUR 169 |
) 4x17 cellogel 200µ (100) |
|
EHCA1200-ST22-100 |
Consort |
ea |
EUR 169 |
) 5.7x14 cellogel 250µ (100) |
|
EHCA1200-ST36-100 |
Consort |
ea |
EUR 160 |
) 5.7x14 cellogel 200µ (100) |
|
EHCA1200-ST37-100 |
Consort |
ea |
EUR 160 |
) 5.7x17 cellogel 250µ (100) |
|
EHCA1200-ST77-100 |
Consort |
ea |
EUR 223 |
) Yeast Mitochondria (S. cerevisiae) |
|
1222-200 |
Biovision |
|
EUR 425 |
|
Yeast Mitochondria (S. cerevisiae) |
|
1222-50 |
Biovision |
|
EUR 207 |
) Yeast Mitochondria (Pichia pastoris) |
|
1111-200 |
Biovision |
200 ug |
EUR 295.2 |
- Highly pure, intact, yeast mitochondria for various studies.
|
|
Description: Mitochondria are the power house of the cells as they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles comprising of an outer membrane and a folded inner membrane called cristae. Isolated mitochondria are a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA, mtRNA, protein profiling, and as a loading control. Highly pure yeast mitochondria are purified using standard protocol as mentioned in the Yeast Mitochondria Isolation Kit - Cat # K259-100 from Pichia pastoris. |
|
Yeast Mitochondria (Pichia pastoris) |
|
1111-50 |
Biovision |
50 ug |
EUR 145.2 |
- Highly pure, intact, yeast mitochondria for various studies.
|
|
Description: Mitochondria are the power house of the cells as they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles comprising of an outer membrane and a folded inner membrane called cristae. Isolated mitochondria are a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA, mtRNA, protein profiling, and as a loading control. Highly pure yeast mitochondria are purified using standard protocol as mentioned in the Yeast Mitochondria Isolation Kit - Cat # K259-100 from Pichia pastoris. |
 Protein) Liver Cytosol (LC-1) Protein |
|
abx160011-200ug |
Abbexa |
200 ug |
EUR 1163 |
- Shipped within 5-10 working days.
|
) AccuBlue NextGen dsDNA Quantitation Kit (1000 assays) |
|
31060 |
Biotium |
1kit |
EUR 459 |
|
Description: Minimum order quantity: 1 unit of 1kit |
) MTT Cell Viability Assay Kit (1000 assays) |
|
30006 |
Biotium |
1KIT |
EUR 204 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
) Resazurin Cell Viability Assay Kit (2500 assays) |
|
30025-1 |
Biotium |
1KIT |
EUR 181 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
 Live Bacterial Gram Stain Kit - 800 assays |
|
32000 |
Biotium |
1kit |
EUR 399 |
|
Description: Minimum order quantity: 1 unit of 1kit |
 Live Bacterial Gram Stain Kit - 200 assays |
|
32000-1 |
Biotium |
1kit |
EUR 190 |
|
Description: Minimum order quantity: 1 unit of 1kit |
 soluble collagen assay kit 2x 96-assays |
|
QZBcol1-2 |
Quickzyme |
2 plates 192 assays |
EUR 614 |
 soluble collagen assay kit 5x 96-assays |
|
QZBcol1-5 |
Quickzyme |
5 plates 480 assays |
EUR 1196 |
 sensitive tissue collagen assay kit 96-assays |
|
QZBtiscol1 |
Quickzyme |
1 plate 96 assays |
EUR 411 |
 sensitive tissue hydroxyproline kit 2x 96-assays |
|
QZBtishyp2 |
Quickzyme |
2 plates 192 assays |
EUR 739 |
 total collagen assay kit 2x 96-assays |
|
QZBtotcol2 |
Quickzyme |
2 plates 192 assays |
EUR 614 |
 total protein assay kit 2x 96-assays |
|
QZBtotprot2 |
Quickzyme |
2 plates 192 assays |
EUR 527 |
 Antibody) bcl-2(100/D5 + 124) Antibody |
|
BNC551234-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5 + 124), CF555 conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5 + 124) Antibody |
|
BNC611234-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5 + 124), CF660R conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5 + 124) Antibody |
|
BNC401234-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5 + 124), CF640R conjugate, Concentration: 0.1mg/mL |
|
bcl-2(100/D5 + 124) Antibody |
|
BNC431234-100 |
Biotium |
100uL |
EUR 199 |
|
Description: Primary antibody against bcl-2(100/D5 + 124), CF543 conjugate, Concentration: 0.1mg/mL |
Most animal fashions of PD, each genetic and toxin-based, goal some part of mitochondrial function to breed points of the human illness. One mannequin that continues to achieve consideration is the MitoPark mouse, created through a cell type-specific knockout of mitochondrial transcription issue A particularly in midbrain DA neurons. RAW264.7 cells have been handled with totally different concentrations of RANKL to distinguish into osteoclasts, and then these cells have been handled with totally different concentrations of CoQ10 with or with out H2 O2 .
S-100 Antibody 
Anti-Mitochondria antibody 
