The purpose of this investigation was to evaluate the recombinant proteins MSA-1, MSA-2c and 12D3 as a combined immunogen for cattle. Fifteen steers were randomly assigned into three groups of five animals each (I, II and III). On day 0, a group I cattle were injected with 50 micrograms of MSA-1, MSA-2c, and r12D3 with Montanide 75 adjuvant; cattle in Group II received PBS-adjuvant, and Group III were untreated controls. On day 14, Group I cattle received a second injection of the three recombinant proteins in adjuvant and Group II cattle again received adjuvant alone. On day 28, all groups of cattle were challenged with a field strain of Babesia Bovis.
After the challenge, the experimental cattle were controlled clinically and serologically. Three of the five steers were immunized with the combined recombinant Bos taurus proteins seroconverted on day 14 post-immunization (P.I.) and the maximum titer was 1:1600. All five immunized steers were strongly seropositive for B. bovis antigens on day 21 P.I. The prepatent periods of vaccinated cattle were delayed until day 10 post-challenge verses 8 and 7 days in Groups II and III, respectively.
Cattle in all groups had fevers above 41 degrees C; the reduction in packed cell volume was not significantly different (P > 0.05) in vaccinated group I compared to groups II and III (29% vs. 26% and 31%, respectively). Treatment was required for one steer in the control group. During the study period, the weight of the cattle in Groups I and II increased by an average of 9 and 7 kg, while the weight of the control cattle decreased by an average of 4 kg. Immunization with MSA-1-MSA-2c-r12D3 proteins was not sufficient to prevent clinical symptoms against challenge, but the immune response was sufficient to protect steers against a mildly virulent strain of B. bovis.
Full product name
Rabbit anti-Bos Taurus (bovine) RETN polyclonal antibody
Uniprot No.: Q762I5
Destination Names: RETN
Alternative Names: RETN antibody; RSTN antibody; resistin antibody
Raised on: Rabbit
Species Reactivity: Bovine
Immunogen: Recombinant bovine resistin protein (19-109AA)
Immunogen Species: Bos taurus (Bovine)
Purification method: >95%, protein G purified
Concentration: Differs from different batches. Please contact us to confirm it.
- Preservative: 0.03% Proclin 300
- Components: 50% glycerol, 0.01 M PBS, pH 7.4
Proven Applications: ELISA
Protocols: ELISA protocol
On receipt, store at -20°C or -80°C. Avoid repeated freezing.
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