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  • Octanoate is differentially metabolized in liver and muscle and fails to rescue cardiomyopathy in CPT2 deficiency
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  • Octanoate is differentially metabolized in liver and muscle and fails to rescue cardiomyopathy in CPT2 deficiency
Octanoate is differentially metabolized in liver and muscle and fails to rescue cardiomyopathy in CPT2 deficiency

Octanoate is differentially metabolized in liver and muscle and fails to rescue cardiomyopathy in CPT2 deficiency

Antibodies, Assay Kits, Biology Cells, cDNA, Clia Kits, DNA Templates, DNA Testing, Elisa Kits, Enzymes, Exosomes, General, Investigating the relationship between mitochondrial genetic variation and cardiovascular-related traits to develop a framework for mitochondrial phenome-wide association studies., Medium & Serums, Microfluidics-enabled phenotyping of a whole population of C. elegans worms over their embryonic and post-embryonic development at single-organism resolution., MSeqDR: A Centralized Knowledge Repository and Bioinformatics Web Resource to Facilitate Genomic Investigations in Mitochondrial Disease., Panel, PCR, Peptides, Recombinant Proteins, Ria Kits, Test Kits, Vector & Virus, Western BlotMarch 30, 2021 Taylor Andrews

Long-chain fatty acid oxidation is steadily impaired in major and systemic metabolic ailments affecting the guts, thus therapeutically growing reliance on usually minor energetic substrates, equivalent to ketones and medium chain fatty acids, may gain advantage cardiac well being. However, the molecular fundamentals of this remedy should not totally recognized. Here, we explored the flexibility of octanoate, an eight-carbon medium-chain fatty acid often known as an unregulated mitochondrial energetic substrate, to ameliorate cardiac hypertrophy in long-chain fatty acid oxidation poor hearts due to carnitine palmitoyltransferase 2 deletion. CPT2 converts acylcarnitines to acyl-CoAs in the mitochondrial matrix for oxidative bioenergetic metabolism.

In Cpt2M-/- mice, excessive octanoate-ketogenic food regimen failed to alleviate myocardial hypertrophy, dysfunction, and acylcarnitine accumulation suggesting that this different substrate is not sufficiently compensatory for vitality provision. Aligning this final result, we recognized a serious metabolic distinction between muscle mass and liver, whereby coronary heart and skeletal muscle mitochondria have been unable to oxidize free octanoate however liver was in a position to oxidize free octanoate. Liver mitochondria, however not coronary heart or muscle, extremely expressed medium-chain acyl-CoA synthetases, probably enabling octanoate activation for oxidation and circumventing acylcarnitine-shuttling.

Conversely, octanoylcarnitine was oxidized by liver, skeletal muscle, and coronary heart, with charges in coronary heart 4-fold larger than liver and, in muscle mass, was not dependent upon CPT2. Together, these knowledge recommend that dietary octanoate can not rescue CPT2-deficient cardiac illness. These knowledge additionally recommend the existence of tissue-specific mechanisms for octanoate oxidative metabolism, with liver being unbiased of free carnitine availability whereas cardiac and skeletal muscle mass rely upon carnitine however not on CPT2. Using high-resolution respirometry and a fluorescent indicator of ROS manufacturing, we then probed for adjustments in: i) lipid- (palmitoylcarnitine-malate), ii) carbohydrate- (pyruvate-malate), and iii) succinate-fueled metabolism, and additionally iv) complicated IV electron switch capability, and v) H2O2 manufacturing.

Oxidative phosphorylation is compromised in hypoxia, however many organisms reside and train in low oxygen environments. Hypoxia-driven diversifications on the mitochondrial degree are frequent and could improve energetic effectivity or reduce deleterious reactive oxygen species (ROS) era. Mitochondria from varied hypoxia-tolerant mammals exhibit sturdy purposeful adjustments following in vivo hypoxia and we hypothesized that comparable plasticity would happen in bare mole-rat skeletal muscle. To check this, we uncovered grownup subordinate bare mole-rats to normoxia (21% O2) or acute (Four h, 7% O2) or persistent hypoxia (4-6 weeks, 11% O2) and then remoted skeletal muscle mitochondria.

Establishment of an in vitro mannequin for analyzing mitochondrial ultrastructure in PRKN-mutated affected person iPSC-derived dopaminergic neurons

Mitochondrial structural adjustments are related to the regulation of mitochondrial operate, apoptosis, and neurodegenerative ailments. PRKN is recognized to be concerned with varied mechanisms of mitochondrial high quality management together with mitochondrial structural adjustments. Parkinson’s illness (PD) with PRKN mutations is characterised by the preferential degeneration of dopaminergic neurons in the substantia nigra pars compacta, which has been prompt to consequence from the buildup of broken mitochondria. However, ultrastructural adjustments of mitochondria particularly in dopaminergic neurons derived from iPSC have not often been analyzed.

To selectively label dopaminergic neurons and analyze mitochondrial morphology on the ultrastructural degree, we generated management and PRKN-mutated affected person tyrosine hydroxylase reporter (TH-GFP) induced pluripotent stem cell (iPSC) traces. Correlative light-electron microscopy evaluation and reside cell imaging of GFP-expressing dopaminergic neurons indicated that iPSC-derived dopaminergic neurons had smaller and much less purposeful mitochondria than these in non-dopaminergic neurons. The fundamental motive for this may be that the dopaminergic neurons can’t be distinguished immediately amongst a mix of iPSC-derived differentiated cells beneath electron microscopy.

Furthermore, the formation of spheroid-shaped mitochondria, which was induced in management dopaminergic neurons by a mitochondrial uncoupler, was inhibited in the PRKN-mutated dopaminergic neurons. These outcomes point out that our established TH-GFP iPSC traces are helpful for characterizing mitochondrial morphology, equivalent to spheroid-shaped mitochondria, in dopaminergic neurons amongst a mix of varied cell sorts. Our in vitro mannequin would offer insights into the vulnerability of dopaminergic neurons and the processes main to the preferential lack of dopaminergic neurons in sufferers with PRKN mutations.

Octanoate is differentially metabolized in liver and muscle and fails to rescue cardiomyopathy in CPT2 deficiency

The Role of Mitophagy in the Regulation of Mitochondrial Energetic Status in Neurons

Mitochondria are the primary mobile vitality powerhouses and provide a lot of the vitality in the type of ATP to gasoline important neuronal features via oxidative phosphorylation (OXPHOS). In Alzheimer illness (AD), metabolic and mitochondrial disruptions are an early characteristic previous any histopathological and scientific manifestations. Mitochondrial malfunction is additionally linked to synaptic defects in early AD. Mitophagy serves as a key mobile high quality management mechanism involving sequestration of broken mitochondria inside autophagosomes and their subsequent degradation in lysosomes.

However, it stays largely unknown whether or not mitophagy is concerned in the regulation of vitality metabolism in neurons, and if that’s the case, whether or not metabolic deficiency in AD is attributed to mitophagy dysfunction. Here we reveal that mitophagy is broadly activated in metabolically enhanced neurons upon OXPHOS stimulation, which sustains excessive energetic exercise by growing mitochondrial turnover and therefore facilitating mitochondrial upkeep. Unexpectedly, in AD-related mutant HsAPP Tg mouse brains, early stimulation of OXPHOS exercise fails to appropriate vitality deficits however exacerbates synapse loss as a consequence of mitophagy failure.

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EUR 401

Oil for Embryo Culturel

YY9305ML100 Bionote 100ml
EUR 85.49
Description: Please check the datasheet of Oil for Embryo Culturel before using the test.

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YY9305ML500 Bionote 500ml
EUR 177.75
Description: Please check the datasheet of Oil for Embryo Culturel before using the test.

Rat Embryo PrimaCell1: Normal Embryonic Chorion Cells

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Rat Embryo PrimaCell2: Normal Fetal Dermal Fibroblasts

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CLC/Ipomoea Medium, Embryo Production

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CLC/Ipomoea Medium, Embryo Production

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EUR 126

Mouse 3T3 (NIH), embryo lysate

MCL-1282 Alpha Diagnostics 100ug
EUR 213

Rat Embryo PrimaCell4: Normal Trophoblast Cells Growth Medium

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Rat Embryo PrimaCell1: Normal Embryonic Chorion Cells Growth Medium

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Human Embryo OptiTDS1: Tissue Dissociation System

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Mouse 3T3 (Balb), murine embryo lysate

MCL-1212 Alpha Diagnostics 100 ug
EUR 213

Rat Embryo FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)

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Rat Ferritin, Mitochondrial (FTMT) Protein

20-abx066551 Abbexa
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
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Total RNA from Rat Normal Tissue: Embryo (RtRAN: 17-19 days)

R1434000-50 Biochain 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Mitochondrial Protein

abx061499-200ug Abbexa 200 ug
EUR 648

Mouse Embryo PrimaCell4: Normal Trophoblast Cells Growth Medium

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Rat Mitochondrial Ribosomal Protein L1 (MRPL1) Protein

20-abx650083 Abbexa
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Uncoupling Protein 1, Mitochondrial (UCP1) Protein

20-abx165898 Abbexa
  • EUR 690.00
  • EUR 286.00
  • EUR 2110.00
  • EUR 815.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
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Rat Uncoupling Protein 1, Mitochondrial (UCP1) Protein

20-abx069601 Abbexa
  • EUR 690.00
  • EUR 286.00
  • EUR 2110.00
  • EUR 815.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Uncoupling Protein 2, Mitochondrial (UCP2) Protein

20-abx069603 Abbexa
  • EUR 759.00
  • EUR 300.00
  • EUR 2374.00
  • EUR 899.00
  • EUR 537.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Topoisomerase I, Mitochondrial (TOP1MT) Protein

20-abx650478 Abbexa
  • EUR 704.00
  • EUR 286.00
  • EUR 2165.00
  • EUR 829.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
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Rat 5'-Nucleotidase, Mitochondrial (NT5M) Protein

20-abx650542 Abbexa
  • EUR 829.00
  • EUR 314.00
  • EUR 2666.00
  • EUR 996.00
  • EUR 578.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Aldehyde Dehydrogenase, Mitochondrial (ALDM) Protein

20-abx652449 Abbexa
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Endonuclease G, Mitochondrial (ENDOG) Protein

20-abx168528 Abbexa
  • EUR 718.00
  • EUR 286.00
  • EUR 2249.00
  • EUR 857.00
  • EUR 509.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
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Mouse Embryo PrimaCell1: Normal Embryonic Chorion Cells Growth Medium

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Human Embryo PrimaCell1: Normal Embryonic Chorion Cells Growth Medium

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Rat Frataxin, mitochondrial (Fxn)

1-CSB-EP009086RA Cusabio
  • EUR 505.00
  • EUR 265.00
  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Rat Frataxin, mitochondrial(Fxn) expressed in E.coli

Rat Frataxin, mitochondrial (Fxn)

1-CSB-EP009086RAe1 Cusabio
  • EUR 621.00
  • EUR 381.00
  • EUR 1943.00
  • EUR 882.00
  • EUR 1335.00
  • EUR 451.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Rat Frataxin, mitochondrial(Fxn) expressed in E.coli

Rat Hexaprenyldihydroxybenzoate methyltransferase, mitochondrial

ELI-32356r Lifescience Market 96 Tests
EUR 886

Rat Mitochondrial Ribosomal Protein L17 ELISA kit

E02M0227-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Mitochondrial Ribosomal Protein L17 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Mitochondrial Ribosomal Protein L17 ELISA kit

E02M0227-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Mitochondrial Ribosomal Protein L17 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Mitochondrial Ribosomal Protein L17 ELISA kit

E02M0227-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Mitochondrial Ribosomal Protein L17 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Mitochondrial Tumor Suppressor 1 (MTUS1) Protein

20-abx650091 Abbexa
  • EUR 662.00
  • EUR 272.00
  • EUR 2040.00
  • EUR 787.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Endonuclease G, Mitochondrial (ENDOG) Protein (Active)

20-abx651325 Abbexa
  • EUR 1121.00
  • EUR 411.00
  • EUR 3724.00
  • EUR 1358.00
  • EUR 759.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) Protein

20-abx167657 Abbexa
  • EUR 704.00
  • EUR 286.00
  • EUR 2165.00
  • EUR 829.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Superoxide Dismutase 2, Mitochondrial (SOD2) Protein

20-abx069197 Abbexa
  • EUR 551.00
  • EUR 244.00
  • EUR 1595.00
  • EUR 648.00
  • EUR 411.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Transcription Factor A, Mitochondrial (TFAM) Protein

20-abx069401 Abbexa
  • EUR 815.00
  • EUR 314.00
  • EUR 2611.00
  • EUR 982.00
  • EUR 578.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Carbonic Anhydrase VB, Mitochondrial (CA5B) Protein

20-abx065700 Abbexa
  • EUR 732.00
  • EUR 286.00
  • EUR 2263.00
  • EUR 871.00
  • EUR 523.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Embryo FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)

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Human Embryo FibrOut3: Fibroblast Inhibitory System (for 500 ml medium)

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Mouse Embryo FibrOut1: Fibroblast Inhibitory System (for 500 ml medium)

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Human Embryo Tissue Preparation Buffer 1: Normal Embryonic Chorion Cells

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Human Embryo Tissue Preparation Buffer 2: Normal Fetal Dermal Fibroblasts

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Rat Embryo PrimaCell4: Normal Trophoblast Cells Growth Supplements with Serum (for 500 ml medium)

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Rat Stress- 70 protein, mitochondrial, Hspa9 ELISA KIT

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Rat Leucine- rich PPR motif- containing protein, mitochondrial,

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Rat Adrenodoxin like protein, mitochondrial(FDX1L) ELISA kit

E02A1855-192T BlueGene 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat Adrenodoxin like protein, mitochondrial(FDX1L) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Adrenodoxin like protein, mitochondrial(FDX1L) ELISA kit

E02A1855-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Rat Adrenodoxin like protein, mitochondrial(FDX1L) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Adrenodoxin like protein, mitochondrial(FDX1L) ELISA kit

E02A1855-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Rat Adrenodoxin like protein, mitochondrial(FDX1L) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Acyl carrier protein, mitochondrial(NDUFAB1) ELISA kit

E02A1930-192T BlueGene 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Acyl carrier protein, mitochondrial(NDUFAB1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Acyl carrier protein, mitochondrial(NDUFAB1) ELISA kit

E02A1930-48 BlueGene 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Acyl carrier protein, mitochondrial(NDUFAB1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Acyl carrier protein, mitochondrial(NDUFAB1) ELISA kit

E02A1930-96 BlueGene 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Acyl carrier protein, mitochondrial(NDUFAB1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Excitingly, lysosomal enhancement in AD neurons restores impaired metabolic operate by selling elimination of broken mitochondria, defending in opposition to synaptic injury in AD mouse brains. Taken collectively, we suggest a brand new mechanism by which mitophagy controls bioenergetic standing in neurons, furthering our understanding of the direct impression of mitophagy defects on AD-linked metabolic deficits and shedding mild on the event of novel therapeutic methods to deal with AD by the early stimulation of mitochondrial metabolism mixed with elevation of lysosomal proteolytic exercise.

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  • Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS

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dna dna ancestry dna block dna bts dna genetics dna hr block dna hrblock login dna lyrics dna motoring dna painter dna productions dna replication dna song dna study slavery dna template sequence dna template slippage dna template strand dna template strand and non-template strand Investigating the relationship between mitochondrial genetic variation and cardiovascular-related traits to develop a framework for mitochondrial phenome-wide association studies. isotopes of hydrogen isotopes of lead isotopes of nitrogen isotopes of oxygen isotopes of oxygen-16 isotopes of rhenium isotopes of sulfur isotopes of uranium isotopes of water isotopes of xenon isotopes of ytterbium isotopes that decay slowly are used to date isotopes website isotypes bio meaning isotypes of antibodies isotypes of antibodies and their function isotypes of immunoglobulin Microfluidics-enabled phenotyping of a whole population of C. elegans worms over their embryonic and post-embryonic development at single-organism resolution. MSeqDR: A Centralized Knowledge Repository and Bioinformatics Web Resource to Facilitate Genomic Investigations in Mitochondrial Disease. ria kids run 2019 ria kista rna definition rna fish rna meaning rna polymerase rna primer

RSS Personal Genomics

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  • Investigating the relationship between mitochondrial genetic variation and cardiovascular-related traits to develop a framework for mitochondrial phenome-wide association studies.
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Categories

  • Antibodies
  • Assay Kits
  • Biology Cells
  • blocking
  • Blog
  • Blot
  • cDNA
  • Clia Kits
  • DNA Templates
  • DNA Testing
  • EIA electrophoresis
  • Elisa Kits
  • Enzymes
  • Exosomes
  • General
  • Guinea
  • Helicobacter
  • Influenza
  • Insect
  • Investigating the relationship between mitochondrial genetic variation and cardiovascular-related traits to develop a framework for mitochondrial phenome-wide association studies.
  • Medium & Serums
  • Microfluidics-enabled phenotyping of a whole population of C. elegans worms over their embryonic and post-embryonic development at single-organism resolution.
  • MSeqDR: A Centralized Knowledge Repository and Bioinformatics Web Resource to Facilitate Genomic Investigations in Mitochondrial Disease.
  • Panel
  • PCR
  • Peptides
  • profile
  • purified
  • Rabbit
  • Rat
  • Recombinant
  • Recombinant Proteins
  • Reindeer
  • Ria Kits
  • Test Kits
  • Vector & Virus
  • Western Blot

Recent Posts

  • Cusabio Bos taurus Recombinant
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  • The role of melatonin in sarcopenia: Advances and application prospects
  • Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS

Tags

dna dna ancestry dna block dna bts dna genetics dna hr block dna hrblock login dna lyrics dna motoring dna painter dna productions dna replication dna song dna study slavery dna template sequence dna template slippage dna template strand dna template strand and non-template strand Investigating the relationship between mitochondrial genetic variation and cardiovascular-related traits to develop a framework for mitochondrial phenome-wide association studies. isotopes of hydrogen isotopes of lead isotopes of nitrogen isotopes of oxygen isotopes of oxygen-16 isotopes of rhenium isotopes of sulfur isotopes of uranium isotopes of water isotopes of xenon isotopes of ytterbium isotopes that decay slowly are used to date isotopes website isotypes bio meaning isotypes of antibodies isotypes of antibodies and their function isotypes of immunoglobulin Microfluidics-enabled phenotyping of a whole population of C. elegans worms over their embryonic and post-embryonic development at single-organism resolution. MSeqDR: A Centralized Knowledge Repository and Bioinformatics Web Resource to Facilitate Genomic Investigations in Mitochondrial Disease. ria kids run 2019 ria kista rna definition rna fish rna meaning rna polymerase rna primer
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