Alzheimer’s Disease Common or sporadic Alzheimer’s disease (AD) occurs late in life, usually after the age of 70, and accounts for the vast majority of the cases. A majority of these late-onset AD patients are associated with primary defects in mitochondrial function. Only about 5% of the AD cases are of the familial variety, which … Read more
Cell lines have become an extremely useful tool for studying protein interactions, gene activation and other biological processes because they represent a more natural biological system than cell-free assays. BPS Bioscience has developed and selected a series of stable cell lines that are suitable for a wide range of research applications. Numerous cell lines are … Read more
CONTENTS Introduction Lysate Preparation Sample Preparation SDS-PAGE Protein Transfer Immunoblotting Detection Buffers and Reagents INTRODUCTION Western blotting makes use of antibodies to determine particular person proteins inside a cell or tissue lysate. Antibodies bind to extremely particular sequences of amino acids, often known as epitopes. Because amino acid sequences fluctuate from protein to protein, western … Read more
Introduction Sandwich ELISA (enzyme-linked immunosorbent assay) entails attachment of a seize antibody to a microplate. Then, samples containing unknown quantity of the goal protein or analyte of curiosity are added and bind to the seize antibody. After washing steps to rid the microplate of unbound substances, an HRP conjugate is added for detection. The ELISA … Read more
The genetic landscape of mitochondrial DNA (mtDNA) has been elusive. By analyzing mtDNA using the whole genome sequence (WGS) of Japanese individuals (n = 1928), we identified 2023 mtDNA variants and high-resolution haplogroups. Frequency spectra of the haplogroups were population-specific and were heterogeneous among geographic regions within Japan. Application of machine learning methods could finely classify the … Read more
MSeqDR is the Mitochondrial Disease Sequence Data Resource, a centralized and complete genome and phenome bioinformatics useful resource constructed by the mitochondrial illness neighborhood to facilitate medical prognosis and analysis investigations of particular person affected person phenotypes, genomes, genes, and variants. A central Web portal (https://mseqdr.org) integrates neighborhood information from expert-curated databases with genomic and phenotype knowledge shared by clinicians and … Read more
The organism Caenorhabditis elegans is a performant mannequin system for finding out human organic processes and illnesses, however till now all phenome information are produced as population-averaged read-outs. Monitoring of particular person responses to drug remedies would nonetheless be extra informative. Here, a new technique to trace totally different phenotypic traits of particular person C. elegans nematodes all through their full … Read more
BACKGROUNDMitochondria play a crucial function in the cell and have DNA impartial of the nuclear genome. There is far proof that mitochondrial DNA (mtDNA) variation performs a function in human well being and illness, nonetheless, this space of investigation has lagged behind analysis into the function of nuclear genetic variation on advanced traits and phenotypic outcomes. … Read more
What is the role of the p53 gene? It does this remains unclear, although the p53 gene is vital for tumor suppression. Upon genotoxic or oncogenic tests, increased p53 activity causes uncontrollable cell cycle arrest, senescence or apoptosis. The 3 cornerstones of those so-called triumvirate. Therefore, it’s long been believed that p53 inhibits tumorigenesis by … Read more
