Western Blot Protocol

CONTENTS Introduction Lysate Preparation Sample Preparation SDS-PAGE Protein Transfer Immunoblotting Detection Buffers and Reagents INTRODUCTION Western blotting makes use of antibodies to determine particular person proteins inside a cell or tissue lysate. Antibodies bind to extremely particular sequences of amino acids, often known as epitopes. Because amino acid sequences fluctuate from protein to protein, western … Read more

Read More

Share

Genetic and phenotypic landscape of the mitochondrial genome in the Japanese population.

The genetic landscape of mitochondrial DNA (mtDNA) has been elusive. By analyzing mtDNA using the whole genome sequence (WGS) of Japanese individuals (n = 1928), we identified 2023 mtDNA variants and high-resolution haplogroups. Frequency spectra of the haplogroups were population-specific and were heterogeneous among geographic regions within Japan. Application of machine learning methods could finely classify the … Read more

Read More

Share

MSeqDR: A Centralized Knowledge Repository and Bioinformatics Web Resource to Facilitate Genomic Investigations in Mitochondrial Disease.

MSeqDR is the Mitochondrial Disease Sequence Data Resource, a centralized and complete genome and phenome bioinformatics useful resource constructed by the mitochondrial illness neighborhood to facilitate medical prognosis and analysis investigations of particular person affected person phenotypes, genomes, genes, and variants. A central Web portal (https://mseqdr.org) integrates neighborhood information from expert-curated databases with genomic and phenotype knowledge shared by clinicians and … Read more

Read More

Share

Microfluidics-enabled phenotyping of a whole population of C. elegans worms over their embryonic and post-embryonic development at single-organism resolution.

The organism Caenorhabditis elegans is a performant mannequin system for finding out human organic processes and illnesses, however till now all phenome information are produced as population-averaged read-outs. Monitoring of particular person responses to drug remedies would nonetheless be extra informative. Here, a new technique to trace totally different phenotypic traits of particular person C. elegans nematodes all through their full … Read more

Read More

Share

Investigating the relationship between mitochondrial genetic variation and cardiovascular-related traits to develop a framework for mitochondrial phenome-wide association studies.

BACKGROUNDMitochondria play a crucial function in the cell and have DNA impartial of the nuclear genome. There is far proof that mitochondrial DNA (mtDNA) variation performs a function in human well being and illness, nonetheless, this space of investigation has lagged behind analysis into the function of nuclear genetic variation on advanced traits and phenotypic outcomes.  … Read more

Read More

Share

P53 research

What is the role of the p53 gene? It does this remains unclear, although the p53 gene is vital for tumor suppression. Upon genotoxic or oncogenic tests, increased p53 activity causes uncontrollable cell cycle arrest, senescence or apoptosis. The 3 cornerstones of those so-called triumvirate. Therefore, it’s long been believed that p53 inhibits tumorigenesis by … Read more

Read More

Share